Programme

Programme

Programme


12th April

Isolation and Reprogramming of Somatic Cells to iPSCs

09:30 – 09:45
Welcome and Introductions
09:45 – 10:45
Reprogramming of Somatic Cells into iPSCs.
Nuria Montserrat, Institute for Bioengineering of Catalonia (IBEC)
10:45 – 11:00
Coffee break
11:00 – 12:00
Strategies promoting pluripotent stem cells differentiation: from monolayers to embryoid bodies.
Elena Garreta, Institute for Bioengineering of Catalonia (IBEC)
12:00 – 13:30
Building Disease Models with human iPSC
Christoph Hoefer, Thermo Fisher Scientific
14:00 – 15:00
Lunch (courtesy of IBEC – Thermo Fisher Scientific)

 

WORKING STATION 1  will provide a small introduction about the different techniques when reprogramming human fibroblasts prior hands on.
10 minutes talk-whole station takes 45 minutes
Transfection of human fibroblasts to iPSCs using Episomal iPSC Reprogramming Vectors.
Gillian Wallace, Thermo Fisher Scientific
Reprogram human fibroblasts to iPSCs using CytoTune™-iPS 2.0 Sendai Reprogramming Kit.
Gillian Wallace, Thermo Fisher Scientific

 

WORKING STATION 2 will provide a small introduction about the different defined cell culture media (E8 versus E8 flex, etc) and the different qualitative assays used as read out for reprogramming.
10 minutes talk whole station takes 45 minutes
Observe and distinguish partially reprogrammed/fully reprogrammed cells cultured in E8 media™.
Elena Garreta, Institute for Bioengineering of Catalonia (IBEC)
Expand iPSCs in vitronectin, human feeders, mouse feeders.Stain iPSCs using APLive staining.
Julie Price, Thermo Fisher Scientific

 

WORKING STATION 3 will provide a small introduction about the different possibilities when differentiation human pluripotent stem cells as monolayer or EB and explain cardiac differentiation.
20 minutes talk whole station takes 45 minutes
Preparation of different media for cardiac differentiation, observation of different time points during the differentiation process and preparation of experiment using iPSCs.
Carmen Hurtado, Institute for Bioengineering of Catalonia (IBEC)

 

13th April

ES & iPS differentiation and genome editing

9:30 – 10:30
Stem cells and organoids.
Laura Batlle, Centre for Genomic Regulation (CRG)
10:30 – 10:45
Coffee break
10:45 – 11:45
Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells.
Stefan Semrau, Leiden University, The Netherlands
11:15–12:45
Characterization of hiPSCs from Wilson disease patients.
Silvia Parisi, University of Naples Federico II, Italy
12:45–13:45
CRISPR Editing: Accelerating innovation and enhancing productivity-CRISPR Design, Delivery and Detection (a case study).
Dr. Livio Loffarelli -Senior Technical Sales Specialist, Synthetic Biology South Europe. Biosciences. Life Science Solutions
14:00–15:00
Lunch (courtesy of IBEC – Thermo Fisher Scientific)

 

WORKING STATION 1 will provide a small introduction about the different techniques when transfecting CAS9 components for genome editing in iPSCs and ESCs following Applied Technologies Brochure (Lipofectamine Stem, Neon Transfection System, Lipofectamine 3000).
Transfection of human pluripotent stem cell lines using the different systems.
Gillian Wallace; Andrea Dawson, Thermo Fisher Scientific

 

WORKING STATION 2 will provide a small introduction about the different defined cell culture media (E8 versus E8 flex, etc) and the different qualitative assays used as read out for reprogramming
10 minutes talk whole station takes 45 minutes
Observe and distinguish iPS clones expanded from the previous day, perform in APLive staining.
Elena Garreta, Institute for Bioengineering of Catalonia (IBEC)

 

WORKING STATION 3 will provide a small introduction about the different possibilities when differentiation human pluripotent stem cells as monolayer or EB and explain endoderm and neural differentiation
20 minutes talk whole station takes 45 minutes
Preparation of different media for endoderm differentiation, observation of different time points during the differentiation process, critical inspection of different time points during the differentiation process.
Carmen Hurtado, Institute for Bioengineering of Catalonia (IBEC)