Programme
12th April
Isolation and Reprogramming of Somatic Cells to iPSCs
09:30 – 09:45 |
Welcome and Introductions |
09:45 – 10:45 |
Reprogramming of Somatic Cells into iPSCs.
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10:45 – 11:00 |
Coffee break |
11:00 – 12:00 |
Strategies promoting pluripotent stem cells differentiation: from monolayers to embryoid bodies.
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12:00 – 13:30 |
Building Disease Models with human iPSC
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14:00 – 15:00 |
Lunch (courtesy of IBEC – Thermo Fisher Scientific) |
WORKING STATION 1 will provide a small introduction about the different techniques when reprogramming human fibroblasts prior hands on.10 minutes talk-whole station takes 45 minutes |
Transfection of human fibroblasts to iPSCs using Episomal iPSC Reprogramming Vectors.
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WORKING STATION 2 will provide a small introduction about the different defined cell culture media (E8 versus E8 flex, etc) and the different qualitative assays used as read out for reprogramming.10 minutes talk whole station takes 45 minutes |
Observe and distinguish partially reprogrammed/fully reprogrammed cells cultured in E8 media™.
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WORKING STATION 3 will provide a small introduction about the different possibilities when differentiation human pluripotent stem cells as monolayer or EB and explain cardiac differentiation.20 minutes talk whole station takes 45 minutes |
Preparation of different media for cardiac differentiation, observation of different time points during the differentiation process and preparation of experiment using iPSCs.
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13th April
ES & iPS differentiation and genome editing
9:30 – 10:30 |
Stem cells and organoids.
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10:30 – 10:45 |
Coffee break |
10:45 – 11:45 |
Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells.
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11:15–12:45 |
Characterization of hiPSCs from Wilson disease patients.
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12:45–13:45 |
CRISPR Editing: Accelerating innovation and enhancing productivity-CRISPR Design, Delivery and Detection (a case study).
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14:00–15:00 |
Lunch (courtesy of IBEC – Thermo Fisher Scientific) |
WORKING STATION 1 will provide a small introduction about the different techniques when transfecting CAS9 components for genome editing in iPSCs and ESCs following Applied Technologies Brochure (Lipofectamine Stem, Neon Transfection System, Lipofectamine 3000). |
Transfection of human pluripotent stem cell lines using the different systems.
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WORKING STATION 2 will provide a small introduction about the different defined cell culture media (E8 versus E8 flex, etc) and the different qualitative assays used as read out for reprogramming10 minutes talk whole station takes 45 minutes |
Observe and distinguish iPS clones expanded from the previous day, perform in APLive staining.
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WORKING STATION 3 will provide a small introduction about the different possibilities when differentiation human pluripotent stem cells as monolayer or EB and explain endoderm and neural differentiation20 minutes talk whole station takes 45 minutes |
Preparation of different media for endoderm differentiation, observation of different time points during the differentiation process, critical inspection of different time points during the differentiation process.
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