AFM BioMed Conference 2025

Atomic Force Microscopy in life sciences and nanomedicine

8^th- 11^thApril 2025 · UB, Barcelona

ORGANIZED BY

CONTACT

afmbiomed@ibecbarcelona.eu

ORGANIZING COMMITTEE

Marina I. Giannotti IBEC, CIBER-BBN, UB

Nuria Gavara, UB

Annalisa Calò, UB

Mireia Oliva, UB

Jean-Luc Pellequer, IBS Grenoble

Felix Rico, Aix-Marseille Univ, INSERM, DyNaMo

Lorena Redondo-Morata, Aix-Marseille Univ, INSERM, DyNaMo

Claire Valotteau (Aix-Marseille Univ., INSERM, DyNaMo)

AFM BioMed Conference 2025

Atomic Force Microscopy in Life Sciences and NanoMedicine

The AFM BioMed Conference focuses on the application of Atomic Force Microscopy (AFM) in life sciences and nanomedicine, exploring how AFM addresses key biological challenges and offers innovative solutions in healthcare.

The conference connects international academic and industry experts in life sciences, bringing together those involved in AFM technology and medical or biological research. It covers a range of length scales, from single molecules, membranes, and cells to multicellular systems and interfaces, integrating imaging and force measurements, and highlights technical developments and the integration of AFM with other analytical techniques. The meeting offers a thorough and insightful overview of advancements in these fields through keynote presentations, lectures, poster sessions, and discussion forums.

#AFMBioMed2025

REGISTRATION CLOSED.

For more information, please contact us at ibecevents@ibecbarcelona.eu

SESSION TOPICS

Single Molecules,
From Membranes to Cells, Multicelluar Systems and Biointerfaces, and AFM Developments

IMPORTANT DEADLINES

Abstract submission: until 15/01/2025

Notification of acceptance: around 10/02/2025

Early registration: until 27/02/2025
Late registration: from 27/02/2025 until 20/03/2025

VENUE

University of Barcelona, Historical Building

Gran Via de les Corts Catalanes, 585
08007 Barcelona

Map | How to get there by public transport

REGISTRATION AND FEES

EARLY BIRD RATE Payment until 27/02

Regular	500€
Students (Master + PhD + Postdoc)	400€
Industry Representatives	800€
Accompanying person	150€

LATE RATE Payment until 20/03

Regular	550€
Students (Master + PhD + Postdoc)	450€
Industry Representatives	850€
Accompanying person	200€

VAT included The registration fee covers attendance to all sessions, lunches, coffee/tea breaks and dinner during the conference and conference materials Conference cancellation policy

SPONSORSHIP OPPORTUNITIES

For any queries about the conference, including opportunities for collaborations or sponsorship, please contact with: ibeccommunications@ibecbarcelona.eu

Or fill in the contact form below and we will be in contact with you shortly:

KEYNOTE SPEAKER:

Małgorzata Lekka

Institute of Nuclear Physics. Polish Academy of Sciences, Krakow

Hans Clevers

Utrecht University, Netherlands

Jamie Davies

The University of Edinburgh, UK

Mo R. Ebrahimkhani

University of Pittsburgh, USA

Arye Elfenbein

Wildtype, USA

INVITED SPEAKER:

Georg Fantner

EPFL – École polytechnique fédérale de Lausanne

Martin Pešl

Masaryk University, Czech Republic

Nancy Forde

Simon Fraser University, Burnaby, Canada

Alexander Cartagena-Rivera

Instituto Nacional de Imágenes Biomédicas y Bioingeniería (NIBIB)

Arye Elfenbein

Wildtype, USA

CHAIRS:

George Heath

University of Leeds, England

Filomena Carvalho

Lisboa University, Portugal

Fernando Moreno-Herero

Centro Nacional de Biotecnología, Madrid

Delphine Sicard

Université Sorbonne Paris Nord

Check the poster presentation list:

– Poster list

– Abstracts book

PROGRAMME:

8th April

9th April

10th April

11th April

8th April

9th April

10th April

11th April

Suggested hotels:

Mesón Castilla Atiram Hotel Barcelona · Carrer de Valldonzella, 5, Ciutat Vella, 08001 Barcelona · Conference discount code: AFM25 (10% off).
Oriente Atiram Hotels Barcelona · Rambla dels Caputxins, 45, 08002 Barcelona · Conference discount code: AFM25 (10% off).
Arenas Atiram Hotel Barcelona · Carrer del Capità Arenas, 20, 08034 Barcelona · Conference discount code: AFM25 (10% off).
Atiram Tres Torres · Carrer de Calatrava, 32, Sarrià-Sant Gervasi, 08017 Barcelona · Conference discount code: AFM25 (10% off).

ABSTRACT SUBMISSION

The conference includes poster sessions and contributed oral presentations where you will have the opportunity to present your work and discuss it with your colleagues.

Please use the following template to submit your abstract.

Abstract submission deadline: until 15/01/2025
Notification of acceptance: around 10/02/2025

SATELLITE PROGRAM

OUTREACH ACTIVITY

8.30h	Registration for the Bruker Workshop at AFM BioMed Conference 2025
9.00h	Bruker Workshop at AFM BioMed Conference 2025
13.30h	Registration for the AFM BioMed Conference 2025
14.20h	Start of the conference · Welcome Jordi Garcia · UB Josep Samitier · IBEC Daniel Navajas Jean Luc Pellequier
15.00h	Exploring cell mechanics: implications for disease and therapy · Malgorzata Lekka Chair: Lorena Redondo-Morata
16.00h	Coffee break + poster session I

16.30h	Validation of contact mechanics models and data analysis approaches for nanoindentation measurements of soft samples · Alessandro Podestà
16.45h	In-Depth Biomechanical Investigation of Liver Sinusoidal Endothelial Cells Cultured on Substrates with Precisely Altered Stiffness Properties · Dibakar Borah
17.00h	Biomechanics of Macrophages During Interaction with Extracellular Stimuli · Massimiliano Galluzzi
17.15h	Ultrathin water layers on viruses and on virus models · Alexander Bittner
17.30h	AFM-based methods to investigate the correlation between macrophage viscoelastic behavior and phenotype in response to substrate stiffness · Livia Angeloni
17.45h	High speed AFM uncovers diffusion of LacY in membranes · Oscar Domenech
18.30h	Welcome Reception at UB Gardens

	FROM MEMBRANES TO CELLS
	Chair: Filomena Carvalho
9.00h	Salbutamol attenuates the arrhythmogenic effect of aminophylline in cardiac organoids experimental model · Martin Pešl
9.30h	Highthroughput Biomechanical Characterization of Macrophage Polarization through Atomic Force Microscopy · Beatriz Cantero Nieto
9.45h	Changes in erythrocyte elasticity and morphology as potential predictors of survival in als · Catarina Sousa Lopes
10.00h	Characterization of the viscoelasticity of Red Blood Cells and rheological analogues with AFM · Agathe Nidriche
10.15h	A different metabolic regulation in favism erythrocytes induces surprising alteration of the cells? properties along the aging · Simone Dinarelli
10.20h	Interfacial Dynamics of Quantum Dot Adsorption on Cholera Vulgaris Cells Wall and Its Impact on Nanomechanical Architecture · Varun Vyas

10.30h	Coffee break + posters I
	Multicellular systems and biointerfaces Chair: Delphine Sicard

11.15h	IRSp53 controls supracellular mechanical properties in epithelial tissues · Alexander Cartagena
11.45h	Characterization of Liquid/PAA and PEO Polymer brushes/Gold interfaces by 1D and 3D-AFM · Dahlia SAAD
12.00h	Scanning probe microscopy and raman spectroscopy investigation of cardiac organoids · Federica Rigoni
12.15h	Multiscale nonlinear mechanics of collagen i hydrogels under stretch · Ignasi Jorba
12.30h	Discovering multifactorial causality using AFM nanoindentation · Jean-Luc Pellequer
12.35h	Correlation between nanomechanical and chemical features of crosslinked hydrogels combining AFM with chemometric methods · Grégory Francius
12.40h	Nanomechanical biomarkers for non-communicable diseases · Andreas Stylianou

13.00h	Lunch + poster session 1

	Single Molecules Chair: Fernando Moreno-Herrero

14.30h	Sequence-dependent flexibility as a probe for biomolecular structure · Nancy Forde
15.00h	AFM studies of the molecular mechanism of genome organization · Je-Kyung Ryu
15.15h	AFM study to reveal the role of sulfation patterns of heparan sulfate in modulating the interactions between human papillomavirus and glycosaminoglycans · Fouzia Bano
15.30h	Interplay of glycans and adsorption-driven deformation in SARS-CoV-2 RBD variants onto surfaces with different hydrophobicity · Antonio Miguel Bosch Fernandez
15.45h	Study of plastocyanin conformational changes with pH by single protein unfolding · Montserrat Sales-Mateo
15.50h	Binding mechanism of oligopeptides on solid surface: Assessing the significance of single-molecule approach · Joanne Lê-Chesnais
15.55h	Atomic Force Microscopy reveals key insights into fibrillar species associated with Alzheimer’s Disease · Panangis Polykretis
16.00h	Coffee break + posters (1)
	AFM developments Chair: George Heath

16.30h	Cellular stepwise confinement reveals different mechanical response in MEC-WT and MEC-KO1 under diverse pharmacological conditions · Riccardo Campanile
16.45h	Mechanical Properties at the Nanoscale of Cardiac Organoids Investigated by Scanning Probe Microscopy · Tommaso Savoldi
17.00h	Nanorheology and Nanoindentation Revealed a Softening and an Increased Viscous Fluidity of Adherent Mammalian Cells upon Increasing the Frequency · Francisco Miguel Espinosa Barea
17.15h	Overcoming challenges in the AFM mechanical nanocharacterization of soft heterogeneous biological materials · Stylianos Vasileios Kontomaris
17.30h	Microscale Biomechanical Characterization of Undecalcified Bone through AFM · Nada Wahman
19:00	External event · El Drapaire

	FROM MEMBRANES TO CELLS Chair: Filomena Carvalho

9.00h	Unlocking the Role of Fibrinogen-Erythrocyte Binding in Cardiovascular Diseases · Filomena Carvalho (chaired by Martin Pešl)
9.30h	Nuclear Stiffening and Structural Alterations Induced by Glycation in Diabetes · Andra Dumitru
9.45h	Nuclear Pore Complex plasticity on intact cell nuclei as seen by AFM · Kassandra Gérard
10.00h	Probing cell nanomechanics with the AFM · Guillaume Lamour
10.15h	Exploring the nanomechanics of Gaucher and Fabry disease models · Marina Placci
10.20h	Fibroblast extracellular-matrix mechanical properties in patients with Mucopolysaccharidosis by Atomic Force Microscopy · Juan Manuel Casares-López

10.30h	Coffee break + posters (2)
	AFM DEVELOPMENTS Chair: George Heath

11.15h	High-Speed Scanning Ion Conductance Microscopy · Georg Fantner
11.45h	Enhancing Large-Scale BioAFM Mechanical Characterization with SmartMapping · Andre Körnig
12.00h	Structural analysis of individual helical filaments by three-dimensional contact point reconstruction atomic force microscopy · Wei-Feng Xue
12.15h	Use of AFM nanoindentation for extracellular vesicle detection and biogenesis analysis: a novel MATLAB-based approach · Federica Collacchi
12.30h	Control Algorithms for Long-Range HS-AFM to Study Leukocyte Tether Formation · Lorenzo Villanueva
12.35h	MEMS based AFM: Towards understanding unfolding dynamics of bacteriorhodopsin in sub-ms time resolution · Suyambulingam Subramanian


13.00h	Lunch + poster session II

	SINGLE MOLECULES Chair: Fernando Moreno-Herrero

14.30h	Exploring viral and long non-coding RNAs secondary structure using Atomic Force Microscopy · Fernando Moreno-Herrero (chaired by N. Forde)
15.00h	Observing Dynamic Conformational Changes within the Coiled-Coil Domain of Different Laminin Isoforms Using High-Speed Atomic Force Microscopy · Franz Clemens
15.15h	Surface active antibiotics in action: a real-time study of the killing mechanism using high-speed atomic force microscopy · Sourav Maity
15.30h	Visualization and analysis of P-glycoprotein dynamics by using HS-AFM · Kanaoka Yui
15.45h	Dynamic Interaction of Trigger Factor with the Ribosome Exit Tunnel During Cotranslational Folding · Eider Nuñez
15.50h	Photothermal infrared nanospectroscopy investigation of alteration in the secondary structure of Aβ1-42 aggregates induced by green tea active compounds · Michal Czaja
15.55h	Lipid membrane remodeling and repair · Adai Colom
16.00h	Coffee break + posters II
	FROM MEMBRANES TO CELLS Chair: Filomena Carvalho

16.30h	High-speed AFM reveals enzyme-induced membrane remodelling · Abeer Alshammari
16.45h	MurG ligase induces fluid regions in bacterial membranes · Ignacio Casuso
17.00h	To adhere or not to adhere: an approach to understand the initial biofilm formation · Hannah Heintz
17.15h	From Single Molecules to Tissues: Correlative BioAFM Serving as Core Facility · Jan Pribyl
17.30h	Unraveling lipid membrane dynamics and phase behavior using AFM · Lorena Redondo-Morata
17.45h	AFM on reconstituted bacterial outer membranes · Corrin Blake
20.00h	Conference Dinner · at Can Travi Nou

	AFM DEVELPOMENTS Chair: George Heath

9.00h	AFM Image Filter Fight Club: Noise Vs Information · George Heath (chaired by Georg Fantner)
9.30h	IR and microwave-Based Techniques for Analysing Molecular Signatures: A Novel Approach to Biomarker Detection · Eric Lesniewska
9.45h	Enhancing atomic force microscopy with optical fibre interferometry · Mário S Rodrigues
10.00h	Implementation of multiparametric corelative atomic force microscopy and optical nanoscopy to study polypharmacy in hepatic endothelium · Bartlomiej Zapotoczny
10.15h	Nanoscale dielectric imaging of cells and bacteria by scanning dielectric microscopy assisted by deep convolutional neural networks · Mauricio Cano Galván
10.20h	Aqueous liquid/solid interface at the nanoscale: a comparison of the 3d-AFM mode to other experimental techniques and simulations · Magali Phaner-Goutorbe

10.30h	Coffee break + posters II
	MULTICELLULAR SYSTEMS AND BIOINTERFACES Chair: Delphine Sicard

11.15h	Mechanobiology of Lung Tissue in Physiological and Pathological Conditions · Delphine Sicard (Chaired by Alexander Cartagena)
11.45h	Exploring the biophysics of P. kessleri and S. cerevisiae cells in coculture using atomic force microscopy for the production of biofuels · Simona Sebastiano
12.00h	Understanding cancer aggregate migration; utilising Bio-AFM, biomechanics and molecular biology at the peritoneal boundary · Jordan Turney
12.15h	Mechanical properties of circulating tumour cells (CTC) and its clusters · Yogesh Saravanan
12.30h	Collagen fibril degradation in thoracic aortic aneurysms · Bojin Marinov
12.35h	Co-cultured aggregates in cylindrical cavities: fibroblasts influence the microenvironment of pancreatic cancer cells · Isis do Vale Meira Lima
13.00h	Lunch



	FROM MEMBRANES TO CELLS Chair: Filomena Carvalho

14.30h	Multifunctional Phenotyping of Individual Cancer Cells Using SICM Probes for Nanoscale Assays and Single-Cell Omics · Petr Gorelkin
14.45h	Key roles of N-terminal capping and fibrillation in the toxicity of phenol soluble modulins secreted by S. aureus · Laura Bonnecaze
15.00h	Transient effects on mechanical properties during cell division of Escherichia coli studied by high-speed in-line force mapping · Christian Ganser
15.15h	Quantitative imaging of bacterial cell wall with AFM: function of PBP1a synthase · Laia Pasquina Lemonche
15.30h	Sialic acid depletion leads to profound remodelling of glycocalyx architecture and mechanics in pancreatic cancer cells · Andrew Massey
15.45h	Effects of microbial glycolipids on phospholipid membranes using Atomic Force Microscopy (AFM) · Yulia Fok
15.50h	Bacterial Adhesion Strength is Linked to Cell Wall Age · Johannes Mischo
16.00h	Coffee break

16.30h	Square table Chair: Felix Rico





17.15	Awards ceremony + concluding remarks

AFM BioMed Conference 2025

Atomic Force Microscopy in life sciences and nanomedicine

8th- 11thApril 2025 · UB, Barcelona

ORGANIZING COMMITTEE

AFM BioMed Conference 2025

Atomic Force Microscopy in Life Sciences and NanoMedicine

SESSION TOPICS

SPONSORed by:

IMPORTANT DEADLINES

VENUE

REGISTRATION AND FEES

SPONSORSHIP OPPORTUNITIES

Małgorzata Lekka

Hans Clevers

Jamie Davies

Mo R. Ebrahimkhani

Arye Elfenbein

Georg Fantner

Martin Pešl

Nancy Forde

Alexander Cartagena-Rivera

Arye Elfenbein

George Heath

Filomena Carvalho

Fernando Moreno-Herero

Delphine Sicard

Check the poster presentation list:

– Poster list

– Abstracts book

FROM MEMBRANES TO CELLS

Multicellular systems and biointerfaces

Single Molecules

AFM developments

FROM MEMBRANES TO CELLS

AFM DEVELOPMENTS

SINGLE MOLECULES

FROM MEMBRANES TO CELLS

AFM DEVELPOMENTS

MULTICELLULAR SYSTEMS AND BIOINTERFACES

FROM MEMBRANES TO CELLS

Square table

Suggested hotels:

ABSTRACT SUBMISSION

SATELLITE PROGRAM

AFM BioMed Conference 2025: Atomic Force Microscopy in life sciences and nanomedicine

MENU

8^th- 11^thApril 2025 · UB, Barcelona